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KMID : 1005220170320020039
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Journal of Embryo Transfer
2017 Volume.32 No. 2 p.39 ~ p.45
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An Increase in Mesenchymal Stem Cells Expressing Nestin in Bone-Marrow-Derived Primary Cells Stimulates Neurogenic Differentiation in Rat
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Han Na-Rae
Lee Hyun
Yun Jung-Im
Kim Choong-Hyo
Hwang Jae-Yeon
Park Kyu-Hyun
Lee Seung-Tae
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Abstract
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Mesenchymal stem cells (MSCs) have been considered an alternative source of neuronal lineage cells, which are difficult to isolate from brain and expand in vitro. Previous studies have reported that MSCs expressing Nestin (Nestin+ MSCs), a neuronal stem/progenitor cell marker, exhibit increased transcriptional levels of neural development-related genes, indicating that Nestin+ MSCs may exert potential with neurogenic differentiation. Accordingly, we investigated the effects of the presence of Nestin+ MSCs in bone-marrow-derived primary cells (BMPCs) on enhanced neurogenic differentiation of BMPCs by identifying the presence of Nestin+ MSCs in uncultured and cultured BMPCs. The percentage of Nestin+ MSCs in BMPCs was measured per passage by double staining with Nestin and CD90, an MSC marker. The efficiency of neurogenic differentiation was compared among passages, revealing the highest and lowest yields of Nestin+ MSCs. The presence of Nestin+ MSCs was identified in BMPCs before in vitro culture, and the highest and lowest percentages of Nestin+ MSCs in BMPCs was observed at the third (P3) and fifth passages (P5). Moreover, significantly the higher efficiency of differentiation into neurons, oligodendrocyte precursor cells and astrocytes was detected in BMPCs at P3, compared with P5. In conclusion, these results demonstrate that neurogenic differentiation can be enhanced by increasing the proportion of Nestin+ MSCs in cultured BMPCs.
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KEYWORD
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Mesenchymal stem cells expressing Nestin , Neurogenic differentiation , Bone marrow , Rat
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